Shopping List

This page contains a compiled list of all parts you will need to buy to build the smfBox, we have split the wavelength dependent components into a separate section: For a section-by-section description of where each part goes visit the overview page.

To buy all the Thorlabs parts together, click the button below to go to the associated cart on the Thorlabs website. Note that you will still need to buy all the non-Thorlabs parts from their respective vendors.
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Standard Parts

Company Product code Quantity Part Nr
Thorlabs AC254-030-AML 1 L2
Thorlabs AC254-100-A-ML 1 L6
Thorlabs AC254-75-B-ML 1 L7
Thorlabs BB1-E02 2 M1/M2
Thorlabs B4CRP/M 1 54
Thorlabs C4W 1 53
Thorlabs C4W-CC 1 57
Thorlabs CF125C/M 4 10/21/30/91
Thorlabs CM1-CC 1 84
Thorlabs CM1-DCH/M 1 25
Thorlabs CWY1 1 60
Thorlabs CXY1 2 78/94
Thorlabs CXYZ05/M 1 63
Thorlabs DC1-DCH/M 1 72
Thorlabs DCC1545M 1 35
Thorlabs DET10A/M 1 42
Thorlabs DFM1/M 1 85
Thorlabs ER1.5-P4 1 24
Thorlabs ER1-P4 1 46
Thorlabs ER3 1 70
Thorlabs ER4-P4 1 64
Thorlabs ER6-P4 1 16
Thorlabs ERSCA 1 71
Thorlabs ERSCA-P4 2 47/64
Thorlabs FFM1 1 55
Thorlabs KCB1/M 1 58
Thorlabs KCB1C/M 2 6/17
Thorlabs RS05P 1 90
Thorlabs RS075P/M 1 88
Thorlabs RS1.5P/M 2 9/20
Thorlabs RS10/M 1 27
Thorlabs RS1P8E 1 29
Thorlabs RS5/M 4 8/19/28/89
Thorlabs RS50/M 2 7/18
Thorlabs SH4S038 1 Screws
Thorlabs SM1A1 1 62
Thorlabs SM1A12 1 52
Thorlabs SM1A3 1 61
Thorlabs SM1A9 1 34
Thorlabs SM1B3 3 41/80/96
Thorlabs SM1CP2 4 56/73/86/87
Thorlabs SM1D12C 1 5
Thorlabs SM1L03 7 11/22/31/36/40/76/92
Thorlabs SM1L03T 1 39
Thorlabs SM1L05 4 37/59/66/67
Thorlabs SM1L10 5 12/14/15/68/69
Thorlabs SM1L20C 1 32
Thorlabs SM1P1 1 4
Thorlabs SM1S10 1 23
Thorlabs SM1T2 4 33/38/77/93
Thorlabs SM1T20 1 13
Thorlabs SM1V10 1 45
Thorlabs SML03 2 79/95
Thorlabs TR30/M 1 74
Thorlabs TR50/M 2 2/44
Thorlabs TR50/M0 1 26
Thorlabs UPH/30/M 1 75
Thorlabs UPH50/M 2 3/43
Thorlabs VC3C/M 1 1
Semrock MM3-311-t6-1 25.4 1 M3
RS Components 168-0758 1 22 AWG Hook Up Wire*
RS Components 526-0241 4 RG-58/U Coaxial Male BNC-Hook Up Wire**
RS Components 886-0633 4 RG-58 Coaxial Male BNC-Male-BNC
RS Components 144-0935 4 Coaxial Female BNC-Female BNC Connector
Piezoconcept FOC.300 1 50
Omicron LightHUB-2 1 515nm and 638nm Lasers
Olympus N5218700 1 Objective oil
Olympus N1480700 1 49
Newport PNH-50 1 P1 (50 µm)
National Instruments PCIe-6353 1 PCI card
National Instruments 186838-02 2 Shielded Cable 68 Pin
Excelitas SPCM-AWRH-14 2 82/98
Edmund 49792 1 L4
Edmund 49793 2 L3/L5
Chroma 21012 1 BS1
Chroma NC395323-T640lpxr 1 DM2
*Any hook up wire of AWG between 28 - 16 can be used
**Need to strip the Croc Clips

Wavelength Dependent Components

These are the wavelength dependent components used in the original smfBox, if you would prefer to excite with / detect a different set of wavelengths then that should be possible, but you will need to change these. Note that whilst the 515 laser cannot excite common green fluorophores as efficiently as a 532, it can be more easily digitally modulated without the use of an AOM, and can also excite fluorophores in the 488 range allowing for a wide choice of labelling.
Part Name Company Product code Quantity Part Nr
Omicron LightHUB®-2 with 515nm 80mW, and 638nm 100mW Omicron LightHUB-2 1 Laser
Excitation Dichroic Chroma ZT532/640rpc 1 DM1
Emission Dichroic Chroma NC395323 - T640lpxr 1 DM2
Donor Emission Filter Semrock FF01-571/72-25 1 F1
Acceptor Emission Filter Semrock FF01-679/41-25 1 F2
Fig 1a. Graph showing the absorbance spectra of two typical fluorophores used with the smfBox, the transmission spectrum of the excitation dichroic which reflects the lasers (shown with dotted lines) into the sample, but permits light emitted from the sample. 1b. Emission spectra of the same dyes (dark lines), with the filters used to clean up the emission (dotted lines). The spectra of the final light permitted to the APD's is shown in solid blocks. In yellow is the donor emission which reaches the acceptor APD.

On casual inspection of the above graph it may appear that greater signal from the donor could be acquired with a longer wavelength bandpass filter. It is worth noting that whilst this is true, it would also permit emission of the raman scatter from the 515 laser, so the bandpass filter used was selected specifically to remove this scatter and lower background.